taqman gene expression assay probes (Thermo Fisher)

Thermo Fisher taqman gene expression assay probes

Genes with known chromatin remodeling functions that were dysregulated according to RNA-seq data from the dmPFC of control versus postdependent rats

Taqman Gene Expression Assay Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/taqman gene expression assay probes/product/Thermo Fisher
Average 90 stars, based on 1 article reviews

taqman gene expression assay probes - by Bioz Stars, 2026-03

90/100 stars

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riz1 (Proteintech)

Proteintech riz1

Riz1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/riz1/product/Proteintech
Average 93 stars, based on 1 article reviews

riz1 - by Bioz Stars, 2026-03

93/100 stars

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prdm2 probe (Advanced Cell Diagnostics Inc)

Advanced Cell Diagnostics Inc prdm2 probe

Prdm2 Probe, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prdm2 probe/product/Advanced Cell Diagnostics Inc
Average 90 stars, based on 1 article reviews

prdm2 probe - by Bioz Stars, 2026-03

90/100 stars

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Image Search Results

Journal: Molecular psychiatry

Article Title: Dependence-induced increase of alcohol self-administration and compulsive drinking mediated by the histone methyltransferase PRDM2

doi: 10.1038/mp.2016.131

Figure Lengend Snippet: Genes with known chromatin remodeling functions that were dysregulated according to RNA-seq data from the dmPFC of control versus postdependent rats

Article Snippet: Inventoried TaqMan Gene expression assay probes (Prdm2: Rn011516793_m1 and Gapdh: Rn4308313; Life Technologies) were used to assess the expression of rat target genes on an ABI 7900HT Fast Real-time PCR System (Life Technologies).

Techniques: Histone Deacetylase Assay

Prdm2 expression in the brain and peripheral organs. ( a ) Human Affymetrix microarray data ( http://www.biogps.org ). ( b and c ) Quantitative real-time PCR (qPCR) of adult mouse tissues. The central nervous system tissue is shown in black bars and the peripheral tissues are shown in gray bars. * P < 0.05.

Journal: Molecular psychiatry

Article Title: Dependence-induced increase of alcohol self-administration and compulsive drinking mediated by the histone methyltransferase PRDM2

doi: 10.1038/mp.2016.131

Figure Lengend Snippet: Prdm2 expression in the brain and peripheral organs. ( a ) Human Affymetrix microarray data ( http://www.biogps.org ). ( b and c ) Quantitative real-time PCR (qPCR) of adult mouse tissues. The central nervous system tissue is shown in black bars and the peripheral tissues are shown in gray bars. * P < 0.05.

Techniques: Expressing, Microarray, Real-time Polymerase Chain Reaction

( a ) Repeated cycles of alcohol intoxication decreased mRNA levels of Prdm2 . The DNA methyltransferase (DNMT) inhibitor RG-108 significantly restored levels of Prdm2 ( n = 6–10;two-way analysis of variance (ANOVA): main effect of group;control versus postdependent;F (1–29) = 6.3; P = 0.02; post hoc analysis control vehicle versus postdependent vehicle; P = 0.008; No significant difference between postdependent RG-108 versus control RG-108 or postdependent RG-108 versus control vehicle). The mean values (± s.e.m.) of control rats are shown in black bars. The mean values (± s.e.m.) of postdependent rats are shown in gray bars. mRNA expression is presented as % change from control/vehicle values. ( b ) Western blot analysis shows that monomethylation at H3K9 (H3K9me1) is significantly reduced in the dorsomedial prefrontal cortex (dmPFC) of postdependent rats ( n = 7 per group;one-way ANOVA;main effect of group;control versus postdependent; F (1–12) = 7.1; P < 0.05). # P < 0.05, control versus postdependent rats.

Journal: Molecular psychiatry

Article Title: Dependence-induced increase of alcohol self-administration and compulsive drinking mediated by the histone methyltransferase PRDM2

doi: 10.1038/mp.2016.131

Figure Lengend Snippet: ( a ) Repeated cycles of alcohol intoxication decreased mRNA levels of Prdm2 . The DNA methyltransferase (DNMT) inhibitor RG-108 significantly restored levels of Prdm2 ( n = 6–10;two-way analysis of variance (ANOVA): main effect of group;control versus postdependent;F (1–29) = 6.3; P = 0.02; post hoc analysis control vehicle versus postdependent vehicle; P = 0.008; No significant difference between postdependent RG-108 versus control RG-108 or postdependent RG-108 versus control vehicle). The mean values (± s.e.m.) of control rats are shown in black bars. The mean values (± s.e.m.) of postdependent rats are shown in gray bars. mRNA expression is presented as % change from control/vehicle values. ( b ) Western blot analysis shows that monomethylation at H3K9 (H3K9me1) is significantly reduced in the dorsomedial prefrontal cortex (dmPFC) of postdependent rats ( n = 7 per group;one-way ANOVA;main effect of group;control versus postdependent; F (1–12) = 7.1; P < 0.05). # P < 0.05, control versus postdependent rats.

Techniques: Expressing, Western Blot

( a–c ) Expression of PRDM2 in rat dorsomedial prefrontal cortex (dmPFC) measured by RNAscope. Red dots show the expression of the neuronal marker RbFox3 , and the green dots show the expression of Prdm2 and blue labeling represents DAPI (4′,6-diamidino-2-phenylindole) staining. ( d ) Prdm2 mRNA expression is decreased after Prdm2 knockdown. ( e ) Site of virus injection. * P < 0.05; Scrambled versus PRDM2.

Journal: Molecular psychiatry

Article Title: Dependence-induced increase of alcohol self-administration and compulsive drinking mediated by the histone methyltransferase PRDM2

doi: 10.1038/mp.2016.131

Figure Lengend Snippet: ( a–c ) Expression of PRDM2 in rat dorsomedial prefrontal cortex (dmPFC) measured by RNAscope. Red dots show the expression of the neuronal marker RbFox3 , and the green dots show the expression of Prdm2 and blue labeling represents DAPI (4′,6-diamidino-2-phenylindole) staining. ( d ) Prdm2 mRNA expression is decreased after Prdm2 knockdown. ( e ) Site of virus injection. * P < 0.05; Scrambled versus PRDM2.

Techniques: Expressing, Marker, Labeling, Staining, Injection

Tripartite approach implicates high confidence targets of PRDM2 in alcohol dependence. ( a ) Chromatin immunoprecipitation-sequencing (ChIP-seq) analysis identified genomic regions subjected to histone 3 at the lysine 9 residue (H3K9me1) epigenetic regulation in control versus postdependent dorsomedial prefrontal cortex (dmPFC). ( b ) Overlay of ChIP-seq and RNA-sequencing (RNA-seq) analysis from control versus postdependent dmPFC identified 119 common genes. These analyses were further overlaid with NanoString gene expression analysis from rat mPFC subjected to PRDM2 short hairpin RNA (shRNA) compared with a scramble control (hypergeometric distribution test P = 0.001). ( c–f ) ChIP-seq data from the four common genes implicated in all three approaches. Top tracks show the UCSC gene relative to the scale shown at the right. The location of the region of differential H3K9me1 enrichment is denoted by the red box below the gene track. The bottom tracks show the Spatial clustering for identification of ChIP-enriched region (SICER)-filtered read enrichment of H3K9me1 ChIP data for the control rats (gray) and postdependent rats (light red) overlaid.

Journal: Molecular psychiatry

Article Title: Dependence-induced increase of alcohol self-administration and compulsive drinking mediated by the histone methyltransferase PRDM2

doi: 10.1038/mp.2016.131

Figure Lengend Snippet: Tripartite approach implicates high confidence targets of PRDM2 in alcohol dependence. ( a ) Chromatin immunoprecipitation-sequencing (ChIP-seq) analysis identified genomic regions subjected to histone 3 at the lysine 9 residue (H3K9me1) epigenetic regulation in control versus postdependent dorsomedial prefrontal cortex (dmPFC). ( b ) Overlay of ChIP-seq and RNA-sequencing (RNA-seq) analysis from control versus postdependent dmPFC identified 119 common genes. These analyses were further overlaid with NanoString gene expression analysis from rat mPFC subjected to PRDM2 short hairpin RNA (shRNA) compared with a scramble control (hypergeometric distribution test P = 0.001). ( c–f ) ChIP-seq data from the four common genes implicated in all three approaches. Top tracks show the UCSC gene relative to the scale shown at the right. The location of the region of differential H3K9me1 enrichment is denoted by the red box below the gene track. The bottom tracks show the Spatial clustering for identification of ChIP-enriched region (SICER)-filtered read enrichment of H3K9me1 ChIP data for the control rats (gray) and postdependent rats (light red) overlaid.

Techniques: ChIP-sequencing, RNA Sequencing Assay, Expressing, shRNA

Overlapping dmPFC gene expression changes caused by a history of alcohol dependence (RNA-seq data from PD versus CTL), as well as knockdown of PRDM2 ( n = 8–9)

Journal: Molecular psychiatry

Article Title: Dependence-induced increase of alcohol self-administration and compulsive drinking mediated by the histone methyltransferase PRDM2

doi: 10.1038/mp.2016.131

Figure Lengend Snippet: Overlapping dmPFC gene expression changes caused by a history of alcohol dependence (RNA-seq data from PD versus CTL), as well as knockdown of PRDM2 ( n = 8–9)

Techniques: Expressing, shRNA

Lentiviral-mediated knockdown of Prdm2 in the dmPFC alters addiction-like behaviors ( a–c ): Voluntary alcohol-self-administration ( a ) (One way ANOVA: main effect of group;scrambled versus PRDM2 rats;F (1,40) = 5.9; P < 0.02), compulsive drinking in a quinine adulteration task ( b ) (Repeated-measure ANOVA: main effect of group;Scrambled versus PRDM2 rats;F (1,48) = 7; P = 0.02), and stress-induced relapse ( c ) (Repeated-measure ANOVA;main effect of group;scrambled versus PRDM2 rats F (1,84) = 9.3; P = 0.006). Lentiviral knockdown of Prdm2 did not affect control behaviors ( d–h ) such as locomotor activity ( d ), average velocity ( e ), sensitivity to shock intensity ( f ), sucrose self-administration ( g ), nor rat weight ( h ). The mean values (± s.e.m.) of scrambled rats are shown in black bars. The mean values (± s.e.m.) of PRDM2 knockdown rats are shown in gray bars ( n = 9–10). * p < 0.05, scrambled versus PRDM2 rats.

Journal: Molecular psychiatry

Article Title: Dependence-induced increase of alcohol self-administration and compulsive drinking mediated by the histone methyltransferase PRDM2

doi: 10.1038/mp.2016.131

Figure Lengend Snippet: Lentiviral-mediated knockdown of Prdm2 in the dmPFC alters addiction-like behaviors ( a–c ): Voluntary alcohol-self-administration ( a ) (One way ANOVA: main effect of group;scrambled versus PRDM2 rats;F (1,40) = 5.9; P < 0.02), compulsive drinking in a quinine adulteration task ( b ) (Repeated-measure ANOVA: main effect of group;Scrambled versus PRDM2 rats;F (1,48) = 7; P = 0.02), and stress-induced relapse ( c ) (Repeated-measure ANOVA;main effect of group;scrambled versus PRDM2 rats F (1,84) = 9.3; P = 0.006). Lentiviral knockdown of Prdm2 did not affect control behaviors ( d–h ) such as locomotor activity ( d ), average velocity ( e ), sensitivity to shock intensity ( f ), sucrose self-administration ( g ), nor rat weight ( h ). The mean values (± s.e.m.) of scrambled rats are shown in black bars. The mean values (± s.e.m.) of PRDM2 knockdown rats are shown in gray bars ( n = 9–10). * p < 0.05, scrambled versus PRDM2 rats.

Techniques: Activity Assay

prdm2 probe Search Results

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